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Welcome to the Oxylipin Profiling Database



Plant oxylipins are products of polyunsaturated fatty acid oxidation and comprise a wide array of molecules, including fatty acid hydroperoxides, divinyl ethers, or the plant hormone, jasmonic acid. The formation of these metabolites occurs by action of specific enzymes or by autoxidation. The enzymatic formation of hydroperoxy fatty acids represents the first step in the synthesis of oxylipins. The hydroperoxides can be converted by enzymes within the so-called oxylipin pathway which seems to be the most prominent pathway of enzymatic lipid peroxidation in plants. The C18 fatty acids, linoleic acid and linolenic acid, as well as the C16 fatty acid, roughanic acid, can be converted by either of the enzymes, a-DOX, 9-LOX, or 13-LOX, giving rise to a multitude of oxylipins. In vivo, oxylipins are involved in abiotic and biotic stress responses. Some oxylipins have direct antimicrobial properties, whereas others may act as regulators of plant defence gene expression.
In order to facilitate navigating the complexity of oxylipin biosynthesis, this web-based interface has been set up for browsing the plant oxylipin pathway. The pathway has been split into separate schemes according to the enzymes involved and the substrates converted (16:3 9-LOX, 16:3 13-LOX, 18:2 9-LOX, etc.). The respective schemes are interactive, and the formation of around 200 oxylipins can be displayed in detail together with mass- and UV-spectral information characteristic for the individual compounds. In addition, the interface is connected to an SQL database available to registered users, which is capable of managing, calculating, and graphically representing data derived from oxylipin profiling experiments. Detailed laboratory protocols are provided to perform the underlying analytical procedures which consist of combinations of HPLC, GC and GC/MS steps that allow the monitoring of more than 150 metabolites of the oxylipin pathway in parallel and in the same sample.
Oxylipin Profiling Database All rights reserved 2004 - 2005 A. Schomburg, Plant Biochemistry, University of Goettingen